abstract：BACKGROUND:Urinary levels of epidermal growth factor to creatinine ratio are considered as an early predictor of interstitial kidney fibrosis but so far no data are available on their biological variation (BV) and derived parameters. The aim of this study is to determine the BV of epidermal growth factor to creatinine ...

journal_title：Clinical laboratory

authors： Segarra A,Martinez C,Carnicer C,Perich C,Jatem E,Martin M

更新日期：2019-11-01 00:00:00

abstract：BACKGROUND:Both the extracellular matrix molecule tenascin-C (Tn-C) and tissue inhibitors of metalloproteinases (TIMPs) have a role in tissue injury, inflammation, and remodeling. In this pilot study, we tried to evaluate the role of these markers in acute kidney injury (AKI). METHODS:A total of 52 subjects were enrol...

journal_title：Clinical laboratory

authors： Ozkan H,Okuturlar Y,Koçoğlu H,Hursitoglu M,Gedikbasi A,Utku İK,Okuturlar O,Dogan H,Serin SO,Harmankaya O,Demir E,Demir B

更新日期：2019-10-01 00:00:00

abstract：BACKGROUND:Although CA 19-9 is the primary marker used in the diagnosis and treatment of pancreatic cancer, other serum tumor markers have also been utilized in the follow-up of pancreatic cancer. We investigated the clinical utility of CYFRA 21-1, AFP, CEA, CA 19-9, CA 125, NSE, and combinations of these markers in pa...

journal_title：Clinical laboratory

authors： Lee JH

更新日期：2019-10-01 00:00:00

abstract：BACKGROUND:To explore the role of resistin (RE) on deep vein thrombosis (DVT) and the molecular mechanism. METHODS:ELISA assay was used to determine the concentration of RE, MMP-2, MMP-9, and PAI-1 in serum of DVT patients and controls. Rats were randomly divided into 4 groups: control, DVT, DVT + RE, and DVT + RE + S...

journal_title：Clinical laboratory

authors： Ding Y,Li X

更新日期：2019-10-01 00:00:00

abstract：BACKGROUND:Multiplex bead assays, also known as addressable laser bead immunoassays (ALBIA) or Luminex® technology, have provided an alternative to enzyme-linked immunoassay, which is still the most widely utilized routine immunoassay for detection of specific autoantibodies. Our laboratory adopted the ALBIA technology...

journal_title：Clinical laboratory

authors： Swana G,Kariyawasam D,Carroll P,Karim MY

更新日期：2019-09-01 00:00:00

abstract：BACKGROUND:Urine sediment parameters of pregnant women are different from those of non-pregnant women, and it is necessary to establish reference intervals for pregnant women. The aim of this study was to establish reference intervals of white blood cell (WBC), red blood cell (RBC), bacteria (BACT), squamous epithelial...

journal_title：Clinical laboratory

authors： Lu S,Han M,Wang D

更新日期：2019-09-01 00:00:00

abstract：BACKGROUND:Genetic markers are routinely used in human identification of paternity, maternity, and kinship cases. We describe a DNA paternity case with one mismatch on SE33 locus between the alleged father (AF) and the child (daughter). Because there was a father-daughter relationship to solve this case we used chromos...

journal_title：Clinical laboratory

authors： Dumache R,Puiu M,Parvanescu R,Rogobete AF,Enache A

更新日期：2019-09-01 00:00:00

abstract：BACKGROUND:To explore the application value of IGRA-ELISA in the diagnosis of tuberculosis. METHODS:A total of 68 tuberculosis and 58 other pulmonary disease case samples were obtained. All the samples were tested by IGRA-ELISA and T-SPOT.TB assay in parallel. The consistency of IGRA-ELISA and T-SPOT.TB in the diagnos...

journal_title：Clinical laboratory

authors： Zhu M,Zhu Z,Yang J,Hu K

更新日期：2019-08-01 00:00:00

abstract：BACKGROUND:Vertigo and acute cerebral infarction (ACI) patients show similar symptoms such as dizziness and imbalance. This study was to investigate the diagnostic values of neutrophil counts and neutrophil to lymphocyte ratio (NLR) in distinguishing patients with ACI from those with vertigo. METHODS:This retrospectiv...

journal_title：Clinical laboratory

authors： Zhang Y,Jiang L,Yang P,Zhang Y

更新日期：2019-08-01 00:00:00

abstract：BACKGROUND:The current study aims to investigate the expression of antisense noncoding RNA in the INK4 locus (ANRIL) in serum of patients with ischemic stroke and its correlation with high-sensitivity C-reactive protein (hs-CRP) and matrix metalloproteinase-9 (MMP-9). METHODS:Real time PCR analysis was carried out to ...

journal_title：Clinical laboratory

authors： Zhang K,Qi M,Yang Y,Xu P,Zhua Y,Zhang J

更新日期：2019-08-01 00:00:00

abstract：BACKGROUND:Reticulocyte count (RET) has been used for many years to estimate the erythropoietic activity of the bone marrow. Fully automated methods not only provide enhanced precision and accuracy, but also enable reliable measurements of mRNA content and cellular indices. However, problems still exist, such as interf...

journal_title：Clinical laboratory

authors： Jiang H,Wang J,Wang K,Gu J,Chen J,Wang Z

更新日期：2019-07-01 00:00:00

abstract：BACKGROUND:Primary neonatal hypocholinesterase is rare; its genetic pattern and mutation still need to be further studied. METHODS:The patient and his parents are studied using next-generation sequencing technology. RESULTS:A boy one day after birth is admitted to the Neonatal Intensive Care Unit at our hospital afte...

journal_title：Clinical laboratory

authors： Lv HY,Yang LH,Bu LN,Wang QL,Gu XL,Wang ZY,Ren PS,Li LX

更新日期：2019-07-01 00:00:00

abstract：BACKGROUND:An updated meta-analysis was performed to clarify the effects of TGF-β1 T869C polymorphism on the risk of diabetic nephropathy (DN) in the Chinese population. METHODS:The studies were searched using PubMed, Springer Link, Ovid, Chinese Wanfang Data Knowledge Ser-vice Platform, Chinese National Knowledge Inf...

journal_title：Clinical laboratory

authors： Zhou D,Mou X,Liu K,Liu W,Xu Y,Zhou D

更新日期：2019-07-01 00:00:00

abstract：BACKGROUND:Ischemia-modified albumin (IMA) derives from naive albumin, modified in the binding region of bivalent ions, as cobalt and iron. The cobalt, released from some types of hip prosthesis seems to be metabolized differently in males and females but the iron ion is more prevalent than cobalt and is detectable in ...

journal_title：Clinical laboratory

authors： Stea S,Pasquale D,Beraudi A,Catalani S,Amabile M,Bracci G,Toni A

更新日期：2019-06-01 00:00:00

abstract：BACKGROUND:The presence and quantification of circulating tumor cells (CTCs) could minimize the mortality among cancer patients by tailored, personalized, and targeted therapy and could also help in the field of investigation about new therapeutic targets. Identification of CTCs has been performed using molecular techn...

journal_title：Clinical laboratory

authors： Mourtzikou A,Stamouli M,Karakitsos P,Athanasas G

更新日期：2019-06-01 00:00:00

abstract：BACKGROUND:In thrombotic thrombocytopenic purpura (TTP), von Willebrand factor (VWF)-platelet rich thrombi form in the microvasculature with ischemia in vital organs. The pathogenesis relates to deficiency of the VWF cleaving protease, ADAMTS-13. METHODS:A case study of a 58-year-old female with previously undiagnosed...

journal_title：Clinical laboratory

authors： Pillay E,Louw S

更新日期：2019-06-01 00:00:00

abstract：BACKGROUND:The aim of the study is to investigate whether programmed death ligand l (PD-L1) on tumor cells has prognostic significance in patients with hepatocellular carcinoma. METHODS:A cohort of 143 patients with hepatocellular carcinoma was enrolled. PD-L1 expression was detected by immunohistochemistry. The assoc...

journal_title：Clinical laboratory

authors： Pei R,Zhang W,Wang S,Huang X,Zou Y

更新日期：2019-05-01 00:00:00

abstract：BACKGROUND:CYP2D6*10 is mainly responsible for the large pharmacokinetic variability of routinely administered metoprolol in middle-aged and elderly Asian patients. Utilizing an efficient method for identifying the CYP2D6*10 genotypes is clinically important for evaluating the pharmacokinetic effect of administration o...

journal_title：Clinical laboratory

authors： Xiang D,Chen C,He S,Li M,Wang C

更新日期：2019-05-01 00:00:00

abstract：:A pregnant 30-year-old female in the 34th gestational week was admitted at University "Maichin Dom" Hospital prior to childbirth. The patient is diagnosed with β-thalassemia. During laboratory screening hemoglobin of 98 g/L was established. Blood smear shows mild microcytic hypochromic anemia: RBC 5.15 x 1012/L, HGB 9...

journal_title：Clinical laboratory

authors： Voleva S,Manolov V,Krumova S,Marinov B,Vasilev V,Shishkov S,Nikolaeva-Glomb L

更新日期：2019-05-01 00:00:00

abstract：BACKGROUND:Estrogens are responsible for the development of the secondary female gender characteristics. Serum estradiol concentration measurements depend widely on automated immunoassay systems such as the Architect i2000SR, Cobas e601, and UniCel DxI 800. Here, we compare the performance of three automated immunoassa...

journal_title：Clinical laboratory

authors： Zhang H,Huang Y,Liang K

更新日期：2019-04-01 00:00:00

abstract：BACKGROUND:Wilms Tumor 1 (WT1) and Survivin genes are important leukemia-associated antigens (LAAs) in AML with potential prognostic impact. METHODS:We investigated WT1 and Survivin expression levels by RT-PCR in 61 AML patients in correlation with clinical characteristics and outcomes. RESULTS:WT1 was overexpressed ...

journal_title：Clinical laboratory

authors： Adnan-Awad S,Meligui YME,Salem SE,Salaheldin O,Ayoub MA,Kamel MM

更新日期：2019-04-01 00:00:00

abstract：BACKGROUND:It is unclear whether hepatitis B virus (HBV) itself causes iron metabolism disorder in patients with chronic hepatitis B (CHB). In this study, we investigated the effect of HBV on iron metabolism at the clinical and cellular levels to determine the pathogenesis of CHB. MATERIALS:We enrolled 41 CHB patients...

journal_title：Clinical laboratory

authors： Hou SW,Wang ZF,Guan SH,Yang K,Wang Q,Chen LW,Zhang H,Wang X,Duan YL,Pan ZL

更新日期：2019-04-01 00:00:00

abstract：:Background: MicroRNA-493 (miR-493) was upregulated in prostate cancer (PCa). This study was designed to investigate the mechanism underlying miR-493 mediated pro-proliferation in PCa cells. Methods: Expression of miR-493 in PCa cell lines (DU145 and PC3) and control cells was determined using qRT-PCR. PC...

journal_title：Clinical laboratory

authors： Deng J,Ma M,Jiang W,Zhang H,Cui S

更新日期：2019-03-01 00:00:00

abstract：BACKGROUND:Its increasing clinical importance has turned 25-hydroxy-Vitamin D (25(OH)D) into an indispensable test in clinical laboratories. In this study, we aimed to analyze the analytical performances of two widely used immunoassays, namely new restandardized Abbott product Architect 25-OH Vitamin D test and the Bec...

journal_title：Clinical laboratory

authors： Yis OM,Bugdayci G,Sönmez ÇA,Yıldız RN

更新日期：2019-03-01 00:00:00

abstract：BACKGROUND:To investigate the distribution of virulence genes and resistance genes in a group of Staphylococcus aureus (S. aureus). METHODS:From February 2013 to April 2014, a total of 39 strains of S. aureus were collected at Hangzhou Yuhang Hospital of Traditional Chinese Medicine, China. Drug sensitivity to 16 kind...

journal_title：Clinical laboratory

authors： Jiang R,Zhu J,Wenig X,Kong H

更新日期：2019-03-01 00:00:00

abstract：BACKGROUND:Rabies is a fatal zoonotic infectious disease, which can be prevented by prompt post-exposure prophylaxis that could be expensive in countries with a large population. The Essen protocol with injection of 5 single doses of human rabies vaccine on separate days is a well-known rabies prophylaxis schedule. Dec...

journal_title：Clinical laboratory

authors： Rahimi P,Vahabpour R,Shirzadi MR,Bahramali G,Aghasadeghi MR

更新日期：2019-01-01 00:00:00

abstract：BACKGROUND:The objective of this study is to evaluate the performance of the GeneXpert MTB/RIF system in Mycobacterium tuberculosis (MTB) diagnosis and the detection of rifampicin resistance in pulmonary and extrapulmonary clinical samples. METHODS:A total of 849 samples (611 pulmonary and 238 extrapulmonary), which w...

journal_title：Clinical laboratory

authors： Terzi HA,Aydemir O,Karakece E,Koroglu M,Altindis M

更新日期：2019-01-01 00:00:00

abstract：BACKGROUND:The onset of acute toxoplasmosis in pregnant women may pose a risk to their growing fetuses. The timely diagnosis of infection in managing the disease and preventing its harmful consequences on the fetus is very important. Therefore, the study was conducted to identify acute toxoplasmosis in the pregnant wom...

journal_title：Clinical laboratory

authors： Sardarian K,Maghsood AH,Farimani M,Hajilooi M,Saidijam M,Ghane ZZ,Mahaki H,Zamani A

更新日期：2019-01-01 00:00:00

abstract：:Background: Many studies have reported higher values of urinary albumin measured by high performance liquid chromatography (HPLC) in comparison with immunochemical methods. The aims of our study were the implementation of the HPLC method for albuminuria, testing the hypothesis about coeluting proteins, comparis...

journal_title：Clinical laboratory

authors： Fortova M,Klapkova E,Sopko B,Prusa R

更新日期：2018-10-31 00:00:00

abstract：BACKGROUND:This study observed and analyzed the internal quality control (IQC) of cystatin C (CysC) so that we can have overall knowledge about imprecision levels in Chinese medical laboratories. METHODS:Using the software developed by the National Center for Clinical Laboratories (NCCL), we can get the IQC informatio...

journal_title：Clinical laboratory

authors： He S,Wang W,Zhao H,Zhang C,He F,Zhong K,Yuan S,Wang Z

更新日期：2018-10-01 00:00:00

abstract：BACKGROUND:Non-obstructive azoospermia (NOA) is an important factor leading to male infertility and the genetic mechanism is not yet clear. It requires investigation to reveal its occurrence based on sequencing technology from the genetic level. Our previous genome wide association study (GWAS) using targeted high-thro...

journal_title：Clinical laboratory

authors： Yang X,Zhang H,Jiang Y,Zhang H,Hu X,Zhu D,Geng D,Liu R

更新日期：2018-10-01 00:00:00

abstract：BACKGROUND:Although colonoscopy-based screening has proven to be highly effective in detecting colorectal cancer (CRC), participation rates remain disappointing. Development of CRC is associated with a number of genetic or somatic mutations. New, non-invasive stool tests are currently being developed based on the detec...

journal_title：Clinical laboratory

authors： Dollinger MM,Behl S,Fleig WE

更新日期：2018-10-01 00:00:00

abstract：BACKGROUND:Chloride is the main anion in human body. A simple and accurate method for serum chloride measurement was developed using ion chromatography (IC) in China. METHODS:In the measurement, serum samples were diluted 500 times with water, filtered and injected into ion chromatography column. A mixed eluent (2 mmo...

journal_title：Clinical laboratory

authors： Han B,Zeng J,Yan Y,Zhao H,Zhang T,Zhou W,Zhang J,Wang J,Wang Y,Zhang C

更新日期：2018-09-01 00:00:00

abstract：:Antiphospholipid syndrome (APS) is an autoimmune disorder associated with arterial/venous thrombosis and pregnancy loss; thrombocytopenia is another common manifestation of APS. In the present study, we discovered a transient ethylenediaminetetraacetic acid-dependent pseudothrombocytopenia (EDTA-PTCP) phenomenon in AP...

journal_title：Clinical laboratory

authors： Bai M,Feng J,Liang G

更新日期：2018-09-01 00:00:00

abstract：BACKGROUND:Cytogenetic and molecular genetic markers have been used recently to stratify risk and predict prognosis of different types of cancers. Macrophage migration inhibitory factor (MIF) is a pro-inflammatory cytokine that plays an important role in the pathogenesis of many diseases, such as autoimmune diseases an...

journal_title：Clinical laboratory

authors： Sharaf-Eldein M,Elghannam D,Elderiny W,Abdel-Malak C

更新日期：2018-09-01 00:00:00

abstract：BACKGROUND:Thymoma is the most common primary anterior mediastinal neoplasm with a high recurrence rate. Long noncoding RNAs (lncRNAs) have recently been indicated to be used as diagnostic and prognostic indicators for different cancers. The aim of this study was to identify new tumor-specific prognostic lncRNA markers...

journal_title：Clinical laboratory

authors： Gong J,Jin S,Pan X,Wang G,Ye L,Tao H,Wen H,Liu Y,Xie Q

更新日期：2018-07-01 00:00:00

abstract：BACKGROUND:The current study aims to evaluate the expression pattern changes of miR-199-3p in diabetic nephropathy (DN) patients and explore whether it could be used as a potential biomarker. METHODS:Real time PCR analysis was performed to examine the level of miR-199-3p in normoalbuminuria group (diabetes mellitus [D...

journal_title：Clinical laboratory

authors： Meng L,Li G,Liu X,Jiang J,Zhu M,Sun Y

更新日期：2018-07-01 00:00:00

abstract：BACKGROUND:The aim of this study was to use a lectin microarray to detect differential glycan profiling of serous glycoprotein in iron overload thalassemia patients. METHODS:This study enrolled iron overload α/β-thalassemia patients and no iron overload α/β-thalassemia patients. Lectin microarray was used to detect th...

journal_title：Clinical laboratory

authors： Huang A,Huang C,Zhang M,Zhu X,Qin X

更新日期：2018-07-01 00:00:00

abstract：BACKGROUND:Staphylococcus aureus (S. aureus) is a versatile pathogen found in many environments and can cause nosocomial infections in the community and hospitals. S. aureus infection is an increasingly serious threat to global public health that requires action across many government bodies, medical and health sectors...

journal_title：Clinical laboratory

authors： Yang CF,Gou WH,Dai XL,Li YM

更新日期：2018-06-01 00:00:00

abstract：BACKGROUND:Cold agglutinin disease is a very rare condition associated with agglutination of erythrocytes in cold environment usually due to IgM type antibodies. Other than hemolytic anemias, it may interfere with routine hemogram tests due to miscalculation of red blood cell count (RBC) and other hemogram parameters c...

journal_title：Clinical laboratory

authors： Erkus E,Kocak MZ,Aktas G,Ozen M,Atak BM,Duman TT,Tekce BK,Savli H

更新日期：2018-06-01 00:00:00